美国耶鲁大学卢俊等研究人员合作发现，细胞表面RNA控制中性粒细胞的招募。2023年1月22日，《细胞》杂志在线发表了这一最新研究成果。
研究人员发现，细胞表面RNA对中性粒细胞的招募至关重要，而哺乳动物的sid-1 RNA转运体同源物是glycoRNA表达所必需的。在小鼠中性粒细胞中很容易检测到细胞表面RNA，消除这些RNA会大大影响中性粒细胞在体内炎症部位的募集，并减少中性粒细胞对内皮细胞的粘附和迁移。
中性粒细胞glycoRNA主要位于细胞表面，对中性粒细胞与内皮细胞的相互作用非常重要，并能被P选择素（Selp）识别。敲除小鼠的Sidt基因可消除中性粒细胞的glycoRNA，并在功能上模拟细胞表面RNA的缺失。这些数据证明了细胞表面glycoRNA的生物学重要性，并强调了RNA介导细胞功能的一个非经典层面。
据介绍，最近在哺乳动物细胞中发现了定位于细胞外表面的RNA，其中包括具有糖修饰的RNA，即glycoRNA。然而，人们对细胞表面RNA的功能意义及其产生还知之甚少。
附：英文原文
Title: Cell surface RNAs control neutrophil recruitment
Author: Ningning Zhang, Wenwen Tang, Lidiane Torres, Xujun Wang, Yasmeen Ajaj, Li Zhu, Yi Luan, Hongyue Zhou, Yadong Wang, Dingyao Zhang, Vadim Kurbatov, Sajid A. Khan, Priti Kumar, Andres Hidalgo, Dianqing Wu, Jun Lu
Issue&Volume: 2023-01-22
Abstract: RNAs localizing to the outer cell surface have been recently identified in mammaliancells, including RNAs with glycan modifications known as glycoRNAs. However, the functionalsignificance of cell surface RNAs and their production are poorly known. We reportthat cell surface RNAs are critical for neutrophil recruitment and that the mammalianhomologs of the sid-1 RNA transporter are required for glycoRNA expression. Cell surface RNAs can be readilydetected in murine neutrophils, the elimination of which substantially impairs neutrophilrecruitment to inflammatory sites in vivo and reduces neutrophils’ adhesion to and migration through endothelial cells. NeutrophilglycoRNAs are predominantly on cell surface, important for neutrophil-endothelialinteractions, and can be recognized by P-selectin (Selp). Knockdown of the murineSidt genes abolishes neutrophil glycoRNAs and functionally mimics the loss of cell surfaceRNAs. Our data demonstrate the biological importance of cell surface glycoRNAs andhighlight a noncanonical dimension of RNA-mediated cellular functions.
DOI: 10.1016/j.cell.2023.12.033
Source: https://www.cell.com/cell/fulltext/S0092-8674(23)01443-5
来源：科学网 小柯机器人