德国慕尼黑工业大学Erwin Grill等研究人员合作发现，拟南芥的耐热性需要温度传感器TWA1。相关论文于2024年5月15日在线发表在《自然》杂志上。
研究人员发现TWA1是一个温度感应转录共调控因子，拟南芥的基础耐热性和获得性耐热性都需要它。在温度升高时，TWA1会改变其构象，并与JASMONATE-ASSOCIATED MYC-LIKE（JAM）转录因子、TOPLESS（TPL）和TOPLESS-RELATED（TPR）蛋白发生物理相互作用，从而形成抑制复合体。TWA1是一种预测的内在无序蛋白，通过氨基末端的高度可变区发挥关键的热感应作用。
温度升高时，TWA1在核亚域聚集，与JAM2和TPL的物理相互作用似乎仅限于这些核亚域。热休克转录因子A2（HSFA2）和热休克蛋白的转录上调依赖于TWA1，而TWA1的同源物提供了不同的温度阈值，这与传感器在热胁迫早期信号传导中的功能一致。植物热传感器的鉴定为通过育种和生物技术调整作物的热适应反应提供了分子工具，也为热遗传学提供了灵敏的温度开关。
研究人员表示，暴露在过高温度下的植物会激活热应激反应，以应对生理挑战并促进长期适应。感知细胞温度以诱导耐热性的机制尚不清楚。
附：英文原文
Title: The temperature sensor TWA1 is required for thermotolerance in Arabidopsis
Author: Bohn, Lisa, Huang, Jin, Weidig, Susan, Yang, Zhenyu, Heidersberger, Christoph, Genty, Bernard, Falter-Braun, Pascal, Christmann, Alexander, Grill, Erwin
Issue&Volume: 2024-05-15
Abstract: Plants exposed to incidences of excessive temperatures activate heat-stress responses to cope with the physiological challenge and stimulate long-term acclimation1,2. The mechanism that senses cellular temperature for inducing thermotolerance is still unclear3. Here we show that TWA1 is a temperature-sensing transcriptional co-regulator that is needed for basal and acquired thermotolerance in Arabidopsis thaliana. At elevated temperatures, TWA1 changes its conformation and allows physical interaction with JASMONATE-ASSOCIATED MYC-LIKE (JAM) transcription factors and TOPLESS (TPL) and TOPLESS-RELATED (TPR) proteins for repressor complex assembly. TWA1 is a predicted intrinsically disordered protein that has a key thermosensory role functioning through an amino-terminal highly variable region. At elevated temperatures, TWA1 accumulates in nuclear subdomains, and physical interactions with JAM2 and TPL appear to be restricted to these nuclear subdomains. The transcriptional upregulation of the heat shock transcription factor A2 (HSFA2) and heat shock proteins depended on TWA1, and TWA1 orthologues provided different temperature thresholds, consistent with the sensor function in early signalling of heat stress. The identification of the plant thermosensors offers a molecular tool for adjusting thermal acclimation responses of crops by breeding and biotechnology, and a sensitive temperature switch for thermogenetics.
DOI: 10.1038/s41586-024-07424-x
Source: https://www.nature.com/articles/s41586-024-07424-x
来源：科学网 小柯机器人