热触发的磷脂翻转稳定质膜的流动性
四川农业大学钦鹏团队在研究中取得进展。他们探明了热触发的磷脂翻转稳定了质膜的流动性。2026年7月1日出版的《自然》发表了这项成果。
本研究表明，在水稻中，P4-ATP酶OsALA5及其β-亚基OsALIS2介导饱和磷脂酰胆碱的热响应性翻转，从而迅速稳定质膜流动性。利用单叶分解脂质组学和互补运输实验，课题组证明了热暴露诱导OsALA5运输活性的分钟时间尺度变化，导致细胞质膜单叶中饱和磷脂酰胆碱的选择性富集。这种OsALA5介导的饱和磷脂酰胆碱翻转可防止热应激时质膜的超流化，从而减轻离子泄漏和细胞死亡。他们对拟南芥和酵母中OsALA5同源物的分析支持质膜定位的快速热相关反应的功能保存，磷脂酰胆碱运输P4-ATP酶。
在多年、多地点的田间试验中，该课题组发现了一种罕见的OsALA5单倍型，它具有耐热性和产量稳定性。他们除了发现P4-ATP酶介导的饱和磷脂酰胆碱在热胁迫下的翻转反应，并为耐热作物的育种提供遗传资源外，他们的研究还揭示了细胞如何在较早阶段对抗热胁迫驱动的质膜超流化，而不是之前已知的转录依赖的脂质重塑反应。
据悉，细胞可以迅速抵消热应力诱导的质膜超流化以防止膜损伤，但细胞如何实现这种早期保护仍不清楚。
附：英文原文
Title: Heat-triggered phospholipid flipping stabilizes plasma membrane fluidity
Author: Fan, Shijun, Gao, Peng, Huang, Kailai, Ying, Wei, Xu, Lei, Chen, Weilan, Ye, Huan, Xi, Yi, Yang, Yan, Qian, Hang, Li, Ting, Tu, Bin, Yuan, Hua, Ma, Bingtian, Wang, Yuping, Zhong, Zhaohui, Xiong, Jiawei, Wang, Hao, Kang, Liangzhu, Tang, Shiwen, Chen, Xuewei, Sun, Linfeng, Xiang, Chengbin, Li, Shigui, Qin, Peng
Issue&Volume: 2026-07-01
Abstract: Cells must rapidly counteract heat stress-induced hyperfluidization of the plasma membrane to prevent membrane damage1,2, yet how cells achieve such early protection remains unknown. Here we show that in rice (Oryza sativa), the P4-ATPase OsALA5, together with its β-subunit OsALIS2, mediates a heat-responsive flipping of saturated phosphatidylcholines that rapidly stabilizes plasma membrane fluidity. Using leaflet-resolved lipidomics and complementary transport assays, we demonstrate that heat exposure induces a minute-timescale shift in OsALA5 transport activity that leads to selective enrichment of saturated phosphatidylcholines in the cytoplasmic plasma membrane leaflet. This OsALA5-mediated saturated phosphatidylcholine flipping prevents plasma membrane hyperfluidization upon heat stress, thus mitigating ion leakage and cell death. Our analyses of OsALA5 orthologues in Arabidopsis thaliana and yeast support functional conservation of a rapid heat-associated response within a subset of plasma membrane-localized, phosphatidylcholine-transporting P4-ATPases. We identified a rare haplotype of OsALA5 that confers both heat tolerance and yield stability in multi-year, multi-location field trials. Thus, beyond identifying this P4-ATPase-mediated flipping of saturated phosphatidylcholines in response to heat stress and providing genetic resources to advance breeding of heat-tolerant crops, our study reveals how cells counteract heat stress-driven plasma membrane hyperfluidization at an earlier stage than the previously known transcription-dependent lipid remodelling response.
DOI: 10.1038/s41586-026-10726-x
Source: https://www.nature.com/articles/s41586-026-10726-x
期刊信息
Nature：《自然》，创刊于1869年。隶属于施普林格·自然出版集团，最新IF：69.504
官方网址：http://www.nature.com/
投稿链接：http://www.nature.com/authors/submit_manuscript.html